Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (6)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Cutfield, J.F.
Right arrow Articles by Cutfield, S.M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cutfield, J.F.
Right arrow Articles by Cutfield, S.M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Protein Engineering, Vol. 13, No. 10, 735-738, October 2000
© 2000 Oxford University Press

Minor structural consequences of alternative CUG codon usage (Ser for Leu) in Candida albicans exoglucanase

J.F. Cutfield1, P.A. Sullivan2 and S.M. Cutfield

Biochemistry Department, School of Medical Sciences, University of Otago, PO Box 56, Dunedin, New Zealand

In some species of Candida the CUG codon is encoded as serine and not leucine. In the case of the exo-ß-1,3-glucanase from the pathogenic fungus C.albicans there are two such translational events, one in the prepro-leader sequence and the other at residue 64. Overexpression of active mature enzyme in a yeast host indicated that these two positions are tolerant to substitution. By comparing the crystal structure of the recombinant protein with that of the native (presented here), it is seen how either serine or leucine can be accommodated at position 64. Examination of the relatively few solved protein structures from C.albicans indicates that other CUG encoded serines are also found at non-essential surface sites. However such codon usage is rare in C.albicans, in contrast to C.rugosa, with direct implications for respective recombinant protein production.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Microbiol. Mol. Biol. Rev.Home page
W. L. Chaffin
Candida albicans Cell Wall Proteins
Microbiol. Mol. Biol. Rev., September 1, 2008; 72(3): 495 - 544.
[Abstract] [Full Text] [PDF]

Home page
 Protein Eng Des SelHome page
C. Suzuki, T. Kashiwagi, and K. Hirayama
Alternative CUG codon usage (Ser for Leu) in Pichia farinosa and the effect of a mutated killer gene in Saccharomyces cerevisiae
Protein Eng. Des. Sel., March 1, 2002; 15(3): 251 - 255.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.