Protein Engineering, Vol. 14, No. 9, 705-710,
September 2001
© 2001 Oxford University Press
Construction of an expression system of insect lysozyme lacking thermal stability: the effect of selection of signal sequence on level of expression in the Pichia pastoris expression system
1 Division of Biological Sciences, Graduate School of Science and 2 Department of Applied Bioscience, Faculty of Agriculture, Hokkaido University, Sapporo 060-0810 and 3 Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Toyama 930-0194, Japan
Expression systems of human and silkworm lysozymes were constructed using the methylotrophic yeast Pichia pastoris as a host. The leader sequence and its prepro peptide of 
-factor (a peptide pheromone derived from yeast) and the native signal sequences of these lysozymes, were used as secretion signals. When the -factor leader is used as the signal sequence, human lysozyme is secreted at a much higher level than is silkworm lysozyme. On the other hand, silkworm lysozyme, when its native signal is used, is secreted more efficiently than human lysozyme. Therefore, we expected that human lysozyme cDNA with a silkworm native signal would be secreted more efficiently than human lysozyme with its native signal. However, its level of expression was not increased. This result indicates that the native signal of silkworm lysozyme does not promote the secretion of the lysozyme, but rather -factor leader inhibits the secretion. Silkworm lysozyme with the -factor leader is so unstable that it could be easily attacked by some proteases and our findings suggest that the level of expression of heterologous protein with signal peptides and its stability are greatly affected by the selection of the appropriate secretion signal sequence.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Kawamura, K. Ohno, M. Ohkuma, Y. Chijiiwa, and T. Torikata Experimental verification of the crucial roles of glu73 in the catalytic activity and structural stability of goose type lysozyme. J. Biochem., July 1, 2006; 140(1): 75 - 85. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kawamura, T. Fukamizo, T. Araki, and T. Torikata Expression of a Synthetic Gene Coding for Ostrich Egg-White Lysozyme in Pichia Pastoris and Its Enzymatic Activity J. Biochem., January 1, 2003; 133(1): 123 - 131. [Abstract] [Full Text] [PDF]
|
|