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PEDS Advance Access originally published online on January 19, 2006
Protein Engineering Design and Selection 2006 19(3):121-128; doi:10.1093/protein/gzj011
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© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Characterization of an Fc{gamma}RI-binding peptide selected by phage display

G. Berntzen1, O.H. Brekke2, S.A. Mousavi1, J.T. Andersen1, T.E. Michaelsen3, T. Berg1, I. Sandlie1,5 and V. Lauvrak1,4

1Department of Molecular Biosciences, University of Oslo, 2Dynal Biotech ASA, Oslo and 3Norwegian Institute of Public Health, Oslo, Norway 4Present address: Akershus University Hospital, Lørenskog, Norway

5 To whom correspondence should be addressed. E-mail address: inger.sandlie{at}imbv.uio.no

The high-affinity IgG receptor, Fc{gamma} receptor I (Fc{gamma}RI), is expressed exclusively on myeloid cells, and there is a great interest in the targeting of vaccine antigens to Fc{gamma}RI using anti-human Fc{gamma}RI antibodies or fragments derived from such molecules. In order to reduce the size and complexity of the targeting reagent, we have searched for Fc{gamma}RI binding peptides in peptide libraries displayed on phage. The human monocytic cell line U937 was used as target. Phages that displayed the consensus peptide CLRSGXGC were selected and revealed increased binding to IFN-{gamma} stimulated versus non-stimulated U937 cells as well as to Fc{gamma}RI transfected versus non-transfected IIA1.6 cells. Furthermore, they bound the extracellular domains of soluble Fc{gamma}RI, but neither Fc{gamma}RIIA, Fc{gamma}RIIB nor Fc{gamma}RIIIB. Binding was inhibited by a synthetic version of the peptide, whereas neither human IgG nor the Fc{gamma}RI-specific monoclonal antibodies (mAb) mAb22 and 32.2 interfered. Flow-cytometry analysis and internalization studies showed that a synthetic biotin-conjugated peptide ADGACLRSGRGCGAAK-bio was able to target U937 cells and Fc{gamma}RI transfected IIA1.6 cells, and further to promote internalization and vesicular degradation of streptavidin coupled to 1 µm magnetic beads. These peptides may have potential as Fc{gamma}RI targeting reagents.

Keywords: cell internalization/Fc{gamma} receptors/peptide selection/phage display/U937 cells

Received July 28, 2005; revised December 12, 2005; accepted December 15, 2005.

Edited by Sally Ward


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