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PEDS Advance Access originally published online on November 30, 2007
Protein Engineering Design and Selection 2007 20(12):577-581; doi:10.1093/protein/gzm060
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© The Author 2007. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Short communication

Selective gene amplification

Bernard T. Kelly1,3 and Andrew D. Griffiths1,2,4

1 Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK 2 Institut de Science et d’Ingénierie Supramoléculaires (ISIS), Université Louis Pasteur, CNRS UMR 7006, 8 allée Gaspard Monge, BP 70028, F-67083 Strasbourg Cedex, France

4 To whom correspondence should be addressed. E-mail: griffiths{at}isis.u-strasbg.fr

We describe a system for directed evolution based on in vitro compartmentalisation in which amplification of a gene is coupled to the formation of product by the enzyme it encodes. This approach mimics the process of natural selection; ‘fitter’ genes — encoding more efficient enzymes — have more ‘offspring’. It allows selection for any activity so long as a product-specific ligand (e.g. an antibody) is available.

Keywords: directed evolution/emulsion/in vitro compartmentalisation/IVC/PCR

Received September 26, 2007; revised September 26, 2007; accepted September 28, 2007.

3 Present address: Cambridge Institute for Medical Research, Hills Road, Cambridge CB2 2XY, UK


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