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PEDS Advance Access originally published online on January 22, 2007
Protein Engineering Design and Selection 2007 20(2):81-90; doi:10.1093/protein/gzl057
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© The Authors 2007. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Antigen selection from an HIV-1 immune antibody library displayed on yeast yields many novel antibodies compared to selection from the same library displayed on phage

D.R. Bowley1, A.F. Labrijn1,2, M.B. Zwick1 and D.R. Burton1,3

1 Departments of Immunology and Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Rd, IMM-2, La Jolla, CA 92037, USA

3 To whom correspondence should be addressed. E-mail: burton{at}scripps.edu

Phage display of antibody libraries has been widely used for over a decade to generate monoclonal antibodies. Yeast display has been developed more recently. Here the two approaches were directly compared using the same HIV-1 immune scFv cDNA library expressed in phage and yeast display vectors and using the same selecting antigen (HIV-1 gp120). Yeast display was shown to sample the immune antibody repertoire considerably more fully than phage display, selecting all the scFv identified by phage display and twice as many novel antibodies. Positive phage display selection appeared to largely reflect those antibodies that as phage-scFv gave the highest signal in phage ELISAs assessing antigen binding. This signal is thought to reflect the efficiency of expression of folded scFv at the phage surface. Increased access to immune repertoires may increase the rescue of novel antibodies of therapeutic or analytical value that often form a minor part of a typical antibody response.

Keywords: HIV-1 antibody response/phage display/scFv/yeast display

Received November 29, 2006; accepted December 6, 2006.

2 Present address: Genmab, Yalelaan 60, 3584 CM Utrecht, The Netherlands


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