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Protein Engineering vol. 4 no. 2 pp. 121-124, 1990
© 1990 Oxford University Press

RESEARCH-ARTICLE

Organization of polar groups of 9 kd calbindin around Ca2+ ions bound to the protein: a microdielectric study

Tomasz A. Wesolowski, Grzegorz Boguta and Gregory A. Andrzej Bierzynski1

University of Warsaw, Institute of Experimental Physics, Department of Biophysics ul.Zwirki i Wigury 93, 02-089 Warszawa 1Department of Biophysics, Institute of Biochemistry and Biophysics, Polish Academy of Sciences ul. Rakowiecka 36, 02-532 Warszawa, Poland

Using a simple model of linear response of polarizable centers to an electric field, function {delta} is defined to characterize structural organization of protein polar groups. The function makes it possible to detect specific structures of nonuniformly distributed and mutually coupled groups that can transmit local structural and charge density perturbations, induced by an ion over long distances to functionally active sites of a protein molecule. In 9 kd calbindin (a small protein from the troponin C superfamily) two structural chains have been demonstrated that link together both Ca2+ ions coordinated by the protein. The chains form a rigid structure stabilized by coordination of one of the ions, so that binding of the other is promoted. Such a structure is probably common to all members of the superfamily and plays an important role in the mechanism of calcium binding by these proteins.

Keywords: 9 kd calbindin/calcium binding/function {delta}/protein polar groups/troponin C superfamily

Received February 19, 1990; accepted July 17, 1990.


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