Protein Engineering, Vol. 12, No. 1, 23-30,
January 1999
© 1999 Oxford University Press
Amino acid composition of protein termini are biased in different manners
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 117984, Russia
An exhaustive statistical analysis of the amino acid sequences at the carboxyl (C) and amino (N) termini of proteins and of coding nucleic acid sequences at the 5' side of the stop codons was undertaken. At the N ends, Met and Ala residues are over-represented at the first (+1) position whereas at positions 2 and 5 Thr is preferred. These peculiarities at N-termini are most probably related to the mechanism of initiation of translation (for Met) and to the mechanisms governing the life-span of proteins via regulation of their degradation (for Ala and Thr). We assume that the C-terminal bias facilitates fixation of the C ends on the protein globule by a preference for charged and Cys residues. The terminal biases, a novel feature of protein structure, have to be taken into account when molecular evolution, three-dimensional structure, initiation and termination of translation, protein folding and life-span are concerned. In addition, the bias of protein termini composition is an important feature which should be considered in protein engineering experiments.
Keywords: : amino acid composition/nucleotide composition/protein structure/protein termini/statistical analysis
1 To whom correspondence should be addressed. E-mail: ber{at}imb.imb.ac.ru
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