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Protein Engineering, Vol. 12, No. 12, 1031-1034, December 1999
© 1999 Oxford University Press

COMMUNICATIONS

Investigation of a tetracycline-regulated phage display system

G. Zahn1, A. Skerra2 and W. Höhne1,3

1 Institut für Biochemie (Charité), Humboldt-Universität zu Berlin, Monbijoustr.2, D-10117 Berlin and 2 Lehrstuhl für Biologische Chemie, Technische Universität München, An der Saatzucht 5, D-85350 Freising-Weihenstephan, Germany

A new vector (pGZ1) was developed for bacterial phage display of antibody fragments using a transcriptional regulation element with tight control. The tetp/°-based phasmid exhibits fully suppressed scFv background synthesis in the absence of inducer and is independent of glucose as a catabolite repressor. The vector is shown to be a useful alternative to commonly used lacp/°-regulated systems.

Keywords: phagemid/pHen/single-chain Fv/tetracycline promoter

3 To whom correspondence should be addressed


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