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Protein Engineering, Vol. 12, No. 8, 701-705, August 1999
© 1999 Oxford University Press

The molecular weight ratio of monomethoxypolyethylene glycol (mPEG) to protein determines the immunotolerogenicity of mPEG proteins

Takanori So, Hiro-O Ito1,2, Yoshiyuki Tsujihata, Masato Hirata1, Tadashi Ueda and Taiji Imoto3

Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, 1 Department of Biochemistry, Kyushu University School of Dentistry, Fukuoka 812-8582 and 2 Department of Preventive Dentistry and Kagoshima University Dental School, Kagoshima 890-8544, Japan

Immunotolerogenic activity of monomethoxypolyethylene glycol- (mPEG) conjugated proteins is a beneficial property in protein pharmaceutics. However, procedures for the preparation of tolerogenic mPEG proteins have not yet been defined. We prepared mPEG proteins with different mPEG contents using three proteins, hen egg lysozyme, ovalbumin and bovine gamma globulin, and their tolerogenicities to antigen-specific T and B cell responses were examined. We found the most appropriate ratio of tolerance induction to be 1.5–2.0, which is the molecular weight ratio of conjugated total mPEGs to protein. This value may assist in the preparation of tolerogenic mPEG proteins.

Keywords: chemical modification/immunological tolerance/monomethoxypolyethylene glycol/neutralizing antibody/protein pharmaceutics

3 To whom correspondence should be addressed


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