Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Crasto, C. J.
Right arrow Articles by Feng, J.-a.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Crasto, C. J.
Right arrow Articles by Feng, J.-a.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Protein Engineering, Vol. 13, No. 5, 309-312, May 2000
© 2000 Oxford University Press

LINKER: a program to generate linker sequences for fusion proteins

Chiquito J. Crasto and Jin-an Feng1

Institute for Cancer Research, Fox Chase Cancer Center, 7701 Burholme Avenue, Philadelphia, PA 19111, USA

The construction of functional fusion proteins often requires a linker sequence that adopts an extended conformation to allow for maximal flexibility. Linker sequences are generally selected based on intuition. Without a reliable selection criterion, the design of such linkers is often difficult, particularly in situations where longer linker sequences are required. Here we describe a program called LINKER which can automatically generate a set of linker sequences that are known to adopt extended conformations as determined by X-ray crystallography and NMR. The only required input to the program is the desired linker sequence length. The program is specifically designed to assist in fusion protein construction. A number of optional input parameters have been incorporated so that users are able to enhance sequence selection based on specific applications. The program output simply contains a set of sequences with a specified length. This program should be a useful tool in both the biotechnology industry and biomedical research. It can be accessed through the Web page http://www.fccc.edu/research/labs/feng/linker.html.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
F. Xue, Z. Gu, and J.-a. Feng
LINKER: a web server to generate peptide sequences with extended conformation
Nucleic Acids Res., July 1, 2004; 32(suppl_2): W562 - W565.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. L. Blazyk and S. J. Lippard
Domain Engineering of the Reductase Component of Soluble Methane Monooxygenase from Methylococcus capsulatus (Bath)
J. Biol. Chem., February 13, 2004; 279(7): 5630 - 5640.
[Abstract] [Full Text] [PDF]

Home page
 Protein Eng Des SelHome page
R. Arai, H. Ueda, A. Kitayama, N. Kamiya, and T. Nagamune
Design of the linkers which effectively separate domains of a bifunctional fusion protein
Protein Eng. Des. Sel., August 1, 2001; 14(8): 529 - 532.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.