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Protein Engineering, Vol. 15, No. 10, 805-809, October 2002
© 2002 Oxford University Press

Robustness of hen lysozyme monitored by random mutations

Kaori Kunichika, Yoshio Hashimoto and Taiji Imoto1

Graduate School of Pharmaceutical Science, Kyushu University,Fukuoka 812-8582, Japan

We investigated the robustness of hen lysozyme by using random mutant libraries. Six random mutant libraries containing 1, 1.5, 2, 3, 5 and 14 amino acid mutations per hen lysozyme were systematically constructed by varying the concentrations of Mg2+ and Mn2+ on polymerase chain reaction. The mutated genes from the six libraries were cloned to a yeast expression vector and a total of 4000 clones were screened on the basis of lysis activity and ELISA employing monoclonal antibody that recognized only lysozyme with native conformation. About 80% of the clones with an average of two amino acid mutations retained active structure. Almost all clones with an average of five mutations lost active structure. On the other hand, 80% of the clones with an average of two amino acid mutations retained both gross conformation and active structure and 24% of the clones with an average of 14 amino acid mutations retained gross conformation. These results show that gross conformation is robust against mutations and so is active structure to a lesser extent.


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