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Protein Engineering vol. 16 no. 12 pp. 865-870, 2003
© 2003 Oxford University Press

COMMUNICATION

The effect of the EAAEAE insert on the property of human metallothionein-3

Qi Zheng1, Wan-Ming Yang1,2, Wen-Hao Yu1, Bin Cai1, Xin-Chen Teng1, Yi Xie3, Hong-Zhe Sun4, Ming-Jie Zhang5 and Zhong-Xian Huang1,6

1Chemical Biology Laboratory, Department of Chemistry and 3Institute of Genetics, Life School, Fudan University, Shanghai 200433, 2Department of Chemistry, Kunming University of Science and Technology, Kunming, 4Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong and 5Department of Biochemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, PR China

6 To whom correspondence should be addressed. e-mail: zxhuang{at}fudan.edu.cn

MT3 shows apparently different properties and function from MT1 even though they have 70% sequence homology. Possibly the two inserts, Thr5 and a negatively charged hexapeptide at position-55 in MT3, play important roles. A series of MT3 variants around the EAAEAE hexapeptide have been prepared by site-directed mutagenesis and their properties and reactivity towards pH, EDTA and DTNB have been studied. Our detailed studies revealed that the EAAEAE insert is essential to the property of MT3. It is the hexapeptide insert, to some extent, making the MT3 {alpha}-domain looser and lower stability of the metal–thiolate cluster, which could be accessed more easily.

Received March 7, 2003; revised October 10, 2003; accepted October 21, 2003


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