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Protein Engineering vol. 16 no. 9 pp. 707-711, 2003
© 2003 Oxford University Press

Stable, soluble T-cell receptor molecules for crystallization and therapeutics

Jonathan M. Boulter1, Meir Glick2, Penio T. Todorov1, Emma Baston1, Malkit Sami1, Pierre Rizkallah3 and Bent K. Jakobsen1,4

1Avidex Ltd. 57 Milton Park, Abingdon, Oxon OX14 4RX, UK, 2 Novartis Pharmaceuticals, One Health Plaza, East Hanover, NJ 07936, USA and 3DARTS, Daresbury Laboratory, Warrington WA4 4AD, UK

4 To whom correspondence should be addressed. e-mail: bent.jakobsen{at}avidex.com

Antibody and T-cell receptors (TCRs) are the primary recognition molecules of the adaptive immune system. Antibodies have been extensively characterized and are being developed for a large number of therapeutic applications. This has been possible because of the ability to manufacture stable, soluble, monoclonal antibodies which retain the antigen specificity of B cells. Unlike antibodies, TCRs are not expressed in a soluble form, but are anchored to the T-cell surface by an insoluble trans-membrane domain. Characterization and development of TCRs has been hampered by the lack of suitable methods for producing them as soluble and stable proteins. Here we report the engineering of soluble human TCRs suitable for crystallization studies and potentially for in vivo therapeutic use.

Received March 11, 2003; revised July 1, 2003; accepted July 30, 2003.


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