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PEDS Advance Access originally published online on February 27, 2004
Protein Engineering Design and Selection 2004 17(3):201-204; doi:10.1093/protein/gzh025
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© 2004 Oxford University Press

COMMUNICATION

A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system

Farid J. Ghadessy and Philipp Holliger1

MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK

1 To whom correspondence should be addressed. e-mail: ph1{at}mrc-lmb.cam.ac.uk

Emulsion formulations used for in vitro compartmentalization (IVC) methods were found to be incompatible with protein expression in the rabbit reticulocyte (RRL) system, causing rapid discoloration and translation shutdown. Here we identify possible causes and describe a novel water-in-oil emulsion which abolished discoloration and allowed high-level in-emulsion expression of active luciferase and human telomerase using the RRL. This novel emulsion greatly expands the range of potential protein targets for IVC.

Received January 22, 2004; accepted February 18, 2004 Edited by Peter Hudson


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