Skip Navigation


PEDS Advance Access originally published online on August 18, 2006
Protein Engineering Design and Selection 2006 19(10):479-481; doi:10.1093/protein/gzl032
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
19/10/479    most recent
gzl032v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Famm, K.
Right arrow Articles by Winter, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Famm, K.
Right arrow Articles by Winter, G.
Related Collections
Right arrow 2006
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Short Communication

Engineering aggregation-resistant proteins by directed evolution

K. Famm1 and G. Winter1,2,3

1 Centre for Protein Engineering, Medical Research Council Centre Hills Road, Cambridge CB2 2QH, UK 2 Laboratory of Molecular Biology, Medical Research Council Centre Hills Road, Cambridge CB2 2QH, UK

3To whom correspondence should be addressed. E-mail address: winter{at}mrc-lmb.cam.c.uk

A method was recently described for selecting aggregation-resistant antibody domains. A repertoire of domains displayed on filamentous bacteriophage were heated/cooled and selected for binding to the affinity ligand protein A specific for the folded domains. Here we describe a generalization of the method based on the selection for retained phage infectivity and for the binding of an appended sequence tag, and applicable to any protein displayable in multivalent form on phage.

Keywords: directed evolution/protein aggregation/phage display

Received July 3, 2006; accepted July 10, 2006.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Protein Eng Des SelHome page
K. Dudgeon, K. Famm, and D. Christ
Sequence determinants of protein aggregation in human VH domains
Protein Eng. Des. Sel., March 1, 2009; 22(3): 217 - 220.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. C. Liu, A. V. Mack, M.-L. Tsao, J. H. Mills, H. S. Lee, H. Choe, M. Farzan, P. G. Schultz, and V. V. Smider
Protein evolution with an expanded genetic code
PNAS, November 18, 2008; 105(46): 17688 - 17693.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.