PEDS Advance Access published online on April 28, 2004
Protein Engineering Design and Selection, doi:10.1093/protein/gzh038
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1 Department of Chemical Engineering & Biological Engineering Division, Massachusetts
Institute of Technology
* To whom correspondence should be addressed. E-mail: wittrup{at}mit.edu.
An scFv has been engineered to bind carcinoembryonic antigen (CEA) with a
dissociation half-time over 4 days at 37°C. Two mutations responsible for this affinity
increase were isolated by screening yeast surface-displayed mutant libraries by flow
cytometry. Soluble expression of the mutant scFv in a yeast secretion system was increased
one hundred-fold by screening mutant libraries for improved yeast surface display level.
This scFv will be useful as a limiting case for evaluating the significance of affinity in tumor targeting to noninternalizing antigens. Keywords:
0
Revised April 2, 2004
Accepted April 8, 2004
Article
Directed Evolution of an Anti-Carcinoembryonic Antigen scFv
with a Four-Day Monovalent Dissociation Half-time at 37°C
2 Royal Free Hospital, University College London
Abstract 
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Connotea 
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