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PEDS Advance Access published online on November 10, 2004
Protein Engineering Design and Selection, doi:10.1093/protein/gzh083
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Received September 30, 2004
Accepted October 13, 2004

Communication

Selection of optical biosensors from chemisynthetic antibody libraries

Laurent Jespers 1, Timothy P. Bonnert 1, and Greg Winter 1*

1 Division for Protein and Nucleic Acid Chemistry, Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK

* To whom correspondence should be addressed.
Greg Winter, E-mail: winter{at}mrc-lmb.cam.ac.uk


  Abstract

We describe a method for creating antibodies with a fluorescent reporter integrated into the antigen-binding site. A reporter molecule was chemically linked to a hypervariable loop of an antibody repertoire displayed on phage, and this repertoire was selected for antigen binding. In one selected antibody, the fluorescence of the probe responded quantitatively to antigen binding. The method may have application for the engineering of homogeneous immunoassays.

Keywords: Antibody repertoire; optical biosensor; chemisynthetic.
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