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PEDS Advance Access published online on November 17, 2004
Protein Engineering Design and Selection, doi:10.1093/protein/gzh088
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Received September 6, 2004
Accepted October 20, 2004

Communication

Improved growth response of antibody/receptor chimera attained by the engineering of transmembrane domain

Masahiro Kawahara 1*, Yuko Ogo 1, Hiroshi Ueda 1, and Teruyuki Nagamune 1

1 Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

* To whom correspondence should be addressed.
Masahiro Kawahara, E-mail: kawahara{at}bio.t.u-tokyo.ac.jp


  Abstract

Structure-based design of antibody/cytokine receptor chimeras has enabled a growth signal transduction in response to non-natural ligands such as fluorescein-conjugated BSA as mimicry of cytokine-cytokine receptor systems. However, while tight on/off regulation is observed in the natural cytokine receptor systems, many chimeras constructed to date showed residual growth-promoting activity in the absence of ligands. Here we try to reduce the basal growth signal intensity from a chimera by engineering the transmembrane domain (TM) that is thought to be involved in the interchain interaction of natural cytokine receptors. When the retroviral vectors encoding the chimeras with either the wild-type erythropoietin receptor (EpoR) TM or the one bearing two mutations in the leucine zipper motif were transduced to non-strictly interleukin-6-dependent 7TD1 cells, a tight antigen-dependent on/off regulation was attained, also demonstrating the first antigen-mediated genetically modified cell amplification of non-strictly factor-dependent cells. The results clearly indicate that the TM mutation is an effective means to improve the growth response of the antibody/receptor chimera.

Keywords: cytokine receptor; antibody; chimeric receptor; transmembrane domain; mutation.
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