PEDS Advance Access published online on October 26, 2005
Protein Engineering Design and Selection, doi:10.1093/protein/gzi059
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1 Laboratory of Molecular Biophysics, The Rex Richards Building, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK; Present address: Department of Chemistry, University of Rome ‘La Sapienza’, Piazzale Aldo Moro 5, I-00185 Rome, Italy
* To whom correspondence should be addressed. The effect of a short loop connecting two transmembrane
Received April 29, 2005
Accepted August 11, 2005
Article
The role of extra-membranous inter-helical loops in helix-helix interactions
2 Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB, Canada T2N 1N4
3 Laboratory of Molecular Biophysics, The Rex Richards Building, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
Mark S. P. Sansom, E-mail: mark{at}biop.ox.ac.uk
Abstract 
-helices was studied using molecular dynamics simulations. Helices F and G from bacteriorhodopsin and two corresponding polyalanine helices were embedded in octane and POPC membranes in a transmembrane configuration both with and without the inter-helical loop. The results indicate that the membrane environment and the sequence of the loop are more influential on the dynamics and structure of the motif than the presence of a loop as such, at least for the time-scales investigated. The four residues in the FG loop are stabilized by four hydrogen bonds. These hydrogen bonds are not present in the polyalanine loop, causing it to be more flexible than the FG loop. This effect was observed independently of the protein environment, stressing the importance of the sequence. The structural analysis indicates that the loop has weak stabilizing properties in all environments. The stabilization due to the presence of the loop was strongest in a simulation of the FG fragment in a membrane-mimetic octane slab. In the simulations of the helix-loop-helix motif embedded in an explicit lipid bilayer model, the lipid bilayer interface compensates to a large extent for the absence of the loop.
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