PEDS Advance Access published online on January 19, 2006
Protein Engineering Design and Selection, doi:10.1093/protein/gzj011
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1 Department of Molecular Biosciences, University of Oslo, Oslo, Norway
* To whom correspondence should be addressed. The high-affinity IgG receptor, Fc
Received July 28, 2005
Revised December 12, 2005
Accepted December 15, 2005
Article
Characterization of an Fc
G. Berntzen 1,
O. H. Brekke 2,
S. A. Mousavi 1,
J. T. Andersen 1,
T. E. Michaelsen 3,
T. Berg 1,
I. Sandlie 1 *,
and
V. Lauvrak 4
RI-binding peptide selected by phage display
2 Dynal Biotech ASA, Oslo, Norway
3 Norwegian Institute of Public Health, Oslo, Norway
4 Department of Molecular Biosciences, University of Oslo, Oslo, Norway; Present address: Akershus University Hospital, Lørenskog, Norway
I. Sandlie, E-mail: inger.sandlie{at}imbv.uio.no
Abstract 
receptor I (FcRI), is expressed exclusively on myeloid cells, and there is a great interest in the targeting of vaccine antigens to FcRI using anti-human FcRI antibodies or fragments derived from such molecules. In order to reduce the size and complexity of the targeting reagent, we have searched for FcRI binding peptides in peptide libraries displayed on phage. The human monocytic cell line U937 was used as target. Phages that displayed the consensus peptide CLRSGXGC were selected and revealed increased binding to IFN- stimulated versus non-stimulated U937 cells as well as to FcRI transfected versus non-transfected IIA1.6 cells. Furthermore, they bound the extracellular domains of soluble FcRI, but neither FcRIIA, FcRIIB nor FcRIIIB. Binding was inhibited by a synthetic version of the peptide, whereas neither human IgG nor the FcRI-specific monoclonal antibodies (mAb) mAb22 and 32.2 interfered. Flow-cytometry analysis and internalization studies showed that a synthetic biotin-conjugated peptide ADGACLRSGRGCGAAK-bio was able to target U937 cells and FcRI transfected IIA1.6 cells, and further to promote internalization and vesicular degradation of streptavidin coupled to 1 µm magnetic beads. These peptides may have potential as FcRI targeting reagents. receptors; peptide selection; phage display; U937 cells.
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