Protein Engineering Design and Selection

COMMENTARIES:

Michael Gait, Janet Thornton, and Ronald Wetzel

Protein Engineering '87—conference report
Protein Eng. 1987 1: 267-270; doi:10.1093/protein/1.4.267 [PDF] [Request Permissions]  

Robin E. Offord

Are these the insulins of the future?
Protein Eng. 1987 1: 271; doi:10.1093/protein/1.4.271 [PDF] [Request Permissions]  

A.R. Rees

Commission of the European Communities: Biotechnology Action Programme (BAP)
Protein Eng. 1987 1: 271-274; doi:10.1093/protein/1.4.271-a [PDF] [Request Permissions]  

REVIEW:

G.Marius Clore and Angela M. Gronenborn

Determination of three-dimensional structures of proteins in solution by nuclear magnetic resonance spectroscopy
Protein Eng. 1987 1: 275-288; doi:10.1093/protein/1.4.275 [PDF] [Request Permissions]  

ORIGINAL ARTICLES:

G. Deléage and B. Roux

An algorithm for protein secondary structure prediction based on class prediction
Protein Eng. 1987 1: 289-294; doi:10.1093/protein/1.4.289 [Abstract] [PDF] [Request Permissions]  

Bruce H. Robinson and Nadrian C. Seeman

The design of a biochip: a self-assembling molecular-scale memory device
Protein Eng. 1987 1: 295-300; doi:10.1093/protein/1.4.295 [Abstract] [PDF] [Request Permissions]  

Henk A. Kelders, Kor H. Kalk, Piet Gros, and Wim G.J. Hol

Automated protein crystallization and a new crystal form of a subtilisin:eglin complex
Protein Eng. 1987 1: 301-303; doi:10.1093/protein/1.4.301 [Abstract] [PDF] [Request Permissions]  

G.Marius Clore, Angela M. Gronenborn, Mogens Kjaer, and Flemming M. Poulsen

The determination of the three-dimensional structure of barley serine proteinase inhibitor 2 by nuclear magnetic resonance, distance geometry and restrained molecular dynamics
Protein Eng. 1987 1: 305-311; doi:10.1093/protein/1.4.305 [Abstract] [PDF] [Request Permissions]  

G.Marius Clore, Angela M. Gronenborn, Michael N.G. James, Mogens Kjaer, Catherine A. McPhalen, and Fleming M. Poulsen

Comparison of the solution and X-ray structures of barley serine proteinase inhibitor 2
Protein Eng. 1987 1: 313-318; doi:10.1093/protein/1.4.313 [Abstract] [PDF] [Request Permissions]  

Brian C. Cunningham and James A. Wells

Improvement in the alkaline stability of subtilisin using an efficient random mutagenesis and screening procedure
Protein Eng. 1987 1: 319-325; doi:10.1093/protein/1.4.319 [Abstract] [PDF] [Request Permissions]  

Takeyoshi Miki, Takanori Yasukochi, Hiroko Nagatani, Masahiro Furuno, Tetsuro Orita, Hidenori Yamada, Taiji Imoto, and Takao Horiuchi

Construction of a plasmid vector for the regulatable high level expression of eukaryotic genes in Escherichia coli: an application to overproduction of chicken lysozyme
Protein Eng. 1987 1: 327-332; doi:10.1093/protein/1.4.327 [Abstract] [PDF] [Request Permissions]  

Taiji Imoto, Hidenori Yamada, Takanori Yasukochi, Eiichi Yamada, Yuji Ito, Tadashi Ueda, Hiroko Nagatani, Takeyoshi Miki, and Tadao Horiuchi

Point mutation of alanine (31) to valine prohibits the folding of reduced lysozyme by sulfhydryl—disulfide interchange
Protein Eng. 1987 1: 333-338; doi:10.1093/protein/1.4.333 [Abstract] [PDF] [Request Permissions]  

Rudolf Moser, Stefan Frey, Karl Münger, Thomas Hehlgans, Stephan Klauser, Hanno Langen, Ernst-L Winnacker, Ronald Mertz, and Bernd Gutte

Expression of the synthetic gene of an artificial DDT-binding polypeptide in Escherichia coli
Protein Eng. 1987 1: 339-343; doi:10.1093/protein/1.4.339 [Abstract] [PDF] [Request Permissions]  

Jacek Biernat, Hanne Hasselmann, Bernd Hofer, Nick Kennedy, and Hubert Köster

The construction and cloning of synthetic genes coding for artificial proteins and expression studies to obtain fusion proteins
Protein Eng. 1987 1: 345-351; doi:10.1093/protein/1.4.345 [Abstract] [PDF] [Request Permissions]  

Jacek Biernat and Hubert Köster

Expression of synthetic genes coding for completely new, nutritionally rich, artificial proteins
Protein Eng. 1987 1: 353-358; doi:10.1093/protein/1.4.353 [Abstract] [PDF] [Request Permissions]  

Vincent C. Emery and David H.L. Bishop

The development of multiple expression vectors for high level synthesis of eukaryotic proteins: expression of LCMV-N and AcNPV polyhedrin protein by a recombinant baculovirus
Protein Eng. 1987 1: 359-366; doi:10.1093/protein/1.4.359 [Abstract] [PDF] [Request Permissions]  

ERRATUM:

D.T. Rogers, E. Hiller, J. Rittenhouse, L. Moberly, I. Edelstein, and F. Marcus

Cloning, expression and site-directed mutagenesis of yeast fructose-1,6-bisphosphatase
Protein Eng. 1987 1: 367; doi:10.1093/protein/1.4.367 [Abstract] [PDF] [Request Permissions]  

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