Protein Engineering Design and Selection

ORIGINAL ARTICLES:

Burkhard Rost

Twilight zone of protein sequence alignments
Protein Eng. 1999 12: 85-94; doi:10.1093/protein/12.2.85 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Asaf A. Salamov, Makiko Suwa, Christine A. Orengo, and Mark B. Swindells

Combining sensitive database searches with multiple intermediates to detect distant homologues
Protein Eng. 1999 12: 95-100; doi:10.1093/protein/12.2.95 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Yuri N. Chirgadze and Elena A. Larionova

Spatial sign-alternating charge clusters in globular proteins
Protein Eng. 1999 12: 101-105; doi:10.1093/protein/12.2.101 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Kuo-Chen Chou and David W. Elrod

Protein subcellular location prediction
Protein Eng. 1999 12: 107-118; doi:10.1093/protein/12.2.107 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

N. Srinivasan, Marcelo Antonelli, Germaine Jacob, Iris Korn, Francisco Romero, Ana Jedlicki, V. Dhanaraj, Muhammed F.-R. Sayed, Tom L. Blundell, Catherine C. Allende, and Jorge E. Allende

Structural interpretation of site-directed mutagenesis and specificity of the catalytic subunit of protein kinase CK2 using comparative modelling
Protein Eng. 1999 12: 119-127; doi:10.1093/protein/12.2.119 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Lakshmanan K. Iyer and Pradman K. Qasba

Molecular dynamics simulation of -lactalbumin and calcium binding c-type lysozyme
Protein Eng. 1999 12: 129-139; doi:10.1093/protein/12.2.129 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Gilles Tiraboschi, Nathalie Jullian, Vincent Thery, Serge Antonczak, Marie-Claude Fournie-Zaluski, and Bernard P. Roques

A three-dimensional construction of the active site (region 507–749) of human neutral endopeptidase (EC.3.4.24.11)
Protein Eng. 1999 12: 141-149; doi:10.1093/protein/12.2.141 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Torsten F. Schwede, Mathias Bädeker, Martin Langer, Janos Rétey, and Georg E. Schulz

Homogenization and crystallization of histidine ammonia-lyase by exchange of a surface cysteine residue
Protein Eng. 1999 12: 151-153; doi:10.1093/protein/12.2.151 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

L.A. Cole, R. Zirngibl, A.W.B. Craig, Z. Jia, and P. Greer

Mutation of a highly conserved aspartate residue in subdomain IX abolishes Fer protein-tyrosine kinase activity
Protein Eng. 1999 12: 155-162; doi:10.1093/protein/12.2.155 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Hsuan-Liang Liu, Clark Ford, and Peter J. Reilly

Mutations to alter Aspergillus awamori glucoamylase selectivity. IV. Combinations of Asn20Cys/Ala27Cys, Ser30Pro, Gly137Ala, 311–314 Loop, Ser411Ala and Ser436Pro
Protein Eng. 1999 12: 163-172; doi:10.1093/protein/12.2.163 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Lena Ågren, Martin Norin, Nils Lycke, and Björn Löwenadler

Hydrophobicity engineering of cholera toxin A1 subunit in the strong adjuvant fusion protein CTA1-DD
Protein Eng. 1999 12: 173-178; doi:10.1093/protein/12.2.173 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

David P. Humphreys, Bryan J. Smith, Lloyd M. King, Shauna M. West, Dominic G. Reeks, and Paul E. Stephens

Efficient site specific removal of a C-terminal FLAG fusion from a Fab' using copper(II) ion catalysed protein cleavage
Protein Eng. 1999 12: 179-184; doi:10.1093/protein/12.2.179 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

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