Protein Engineering Design and Selection

ORIGINAL ARTICLES:

Narayanan Eswar and C. Ramakrishnan

Deterministic features of side-chain main-chain hydrogen bonds in globular protein structures
Protein Eng. 2000 13: 227-238; doi:10.1093/protein/13.4.227 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

B.O. Brandsdal and A.O. Smalås

Evaluation of protein–protein association energies by free energy perturbation calculations
Protein Eng. 2000 13: 239-245; doi:10.1093/protein/13.4.239 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Tuomo Laitinen, Juha Rouvinen, and Mikael Peräkylä

Inversion of the roles of the nucleophile and acid/base catalysts in the covalent binding of epoxyalkyl xyloside inhibitor to the catalytic glutamates of endo-1,4-ß-xylanase (XYNII): a molecular dynamics study
Protein Eng. 2000 13: 247-252; doi:10.1093/protein/13.4.247 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Zeily Nurachman, Satoshi Akanuma, Takao Sato, Tairo Oshima, and Nobuo Tanaka

Crystal structures of 3-isopropylmalate dehydrogenases with mutations at the C-terminus: crystallographic analyses of structure–stability relationships
Protein Eng. 2000 13: 253-258; doi:10.1093/protein/13.4.253 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Dinlaka Sriprapundh, Claire Vieille, and J.Gregory Zeikus

Molecular determinants of xylose isomerase thermal stability and activity: analysis of thermozymes by site-directed mutagenesis
Protein Eng. 2000 13: 259-265; doi:10.1093/protein/13.4.259 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Annie Savoie, François Sanschagrin, Timothy Palzkill, Normand Voyer, and Roger C. Levesque

Structure–function analysis of -helix H4 using PSE-4 as a model enzyme representative of class A ß-lactamases
Protein Eng. 2000 13: 267-274; doi:10.1093/protein/13.4.267 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Thomas Lanio, Albert Jeltsch, and Alfred Pingoud

On the possibilities and limitations of rational protein design to expand the specificity of restriction enzymes: a case study employing EcoRV as the target
Protein Eng. 2000 13: 275-281; doi:10.1093/protein/13.4.275 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Dahai Tang, Shoji Ando, Yozo Takasaki, and Jutaro Tadano

Mutational analyses of restriction endonuclease—HindIII mutant E86K with higher activity and altered specificity
Protein Eng. 2000 13: 283-289; doi:10.1093/protein/13.4.283 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

D. Chaillot, N. Declerck, K. Niefind, D. Schomburg, T. Chardot, and J.C. Meunier

Mutation of recombinant catalytic subunit of the protein kinase CK2 that affects catalytic efficiency and specificity
Protein Eng. 2000 13: 291-298; doi:10.1093/protein/13.4.291 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Shuichiro Goda, Kazufumi Takano, Yuriko Yamagata, Yoshio Katakura, and Katsuhide Yutani

Effect of extra N-terminal residues on the stability and folding of human lysozyme expressed in Pichia pastoris
Protein Eng. 2000 13: 299-307; doi:10.1093/protein/13.4.299 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

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