Protein Engineering Design and Selection

ORIGINAL ARTICLES:

Tetsuya Hori, Hideaki Moriyama, Jitsutaro Kawaguchi, Yoko Hayashi-Iwasaki, Tairo Oshima, and Nobuo Tanaka

The initial step of the thermal unfolding of 3-isopropylmalate dehydrogenase detected by the temperature-jump Laue method
Protein Eng. 2000 13: 527-533; doi:10.1093/protein/13.8.527 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Jongsun Jung and Byungkook Lee

Protein structure alignment using environmental profiles
Protein Eng. 2000 13: 535-543; doi:10.1093/protein/13.8.535 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Andreas Prlic, Francisco S. Domingues, and Manfred J. Sippl

Structure-derived substitution matrices for alignment of distantly related sequences
Protein Eng. 2000 13: 545-550; doi:10.1093/protein/13.8.545 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

S. Chakraborty, S. Bhattacharya, S. Ghosh, A.K. Bera, U. Haldar, A.K. Pal, B.P. Mukhopadhyay, and Asok Banerjee

Structural and interactional homology of clinically potential trypsin inhibitors: molecular modelling of Cucurbitaceae family peptides using the X-ray structure of MCTI-II
Protein Eng. 2000 13: 551-555; doi:10.1093/protein/13.8.551 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Shido Kawase, Sung-Woo Cho, James Rozelle, Robert M. Stroud, Janet Finer-Moore, and Daniel V. Santi

Replacement set mutagenesis of the four phosphate-binding arginine residues of thymidylate synthase
Protein Eng. 2000 13: 557-563; doi:10.1093/protein/13.8.557 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Olan Dolezal, Lesley A. Pearce, Lynne J. Lawrence, Airlie J. McCoy, Peter J. Hudson, and Alexander A. Kortt

ScFv multimers of the anti-neuraminidase antibody NC10: shortening of the linker in single-chain Fv fragment assembled in V_L to V_H orientation drives the formation of dimers, trimers, tetramers and higher molecular mass multimers
Protein Eng. 2000 13: 565-574; doi:10.1093/protein/13.8.565 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Arthur E. Frankel, Jason Ramage, Melanie Kiser, Richard Alexander, Gregory Kucera, and Mark Steven Miller

Characterization of diphtheria fusion proteins targeted to the human interleukin-3 receptor
Protein Eng. 2000 13: 575-581; doi:10.1093/protein/13.8.575 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Shin-ichi Takemura, Ryutaro Asano, Kouhei Tsumoto, Shinji Ebara, Naoki Sakurai, Yu Katayose, Hideaki Kodama, Hiroshi Yoshida, Masanori Suzuki, Kohzoh Imai, Seiki Matsuno, Toshio Kudo, and Izumi Kumagai

Construction of a diabody (small recombinant bispecific antibody) using a refolding system
Protein Eng. 2000 13: 583-588; doi:10.1093/protein/13.8.583 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Valery A. Petrenko and George P. Smith

Phages from landscape libraries as substitute antibodies
Protein Eng. 2000 13: 589-592; doi:10.1093/protein/13.8.589 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Laura Marrone, Kelly A. McAllister, and Anthony J. Clarke

Characterization of function and activity of domains A, B and C of xylanase C from Fibrobacter succinogenes S85
Protein Eng. 2000 13: 593-601; doi:10.1093/protein/13.8.593 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

CORRIGENDUM:

T. Ueda, K. Masumoto, R. Ishibashi, T. So, and T. Imoto

Remarkable thermal stability of doubly intramolecularly cross-linked hen lysosyme Protein Engineering (2000), 3, 193–196
Protein Eng. 2000 13: 602. [Extract] [FREE Full Text] [PDF] [Request Permissions]  

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