Protein Engineering Design and Selection

COMMUNICATION:

Andelko Hrzenjak, Andreas Artl, Gabriele Knipping, Gerhard Kostner, Wolfgang Sattler, and Ernst Malle

Silent mutations in secondary Shine–Dalgarno sequences in the cDNA of human serum amyloid A4 promotes expression of recombinant protein in Escherichia coli
Protein Eng. 2001 14: 949-952; doi:10.1093/protein/14.12.949 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

ORIGINAL ARTICLES:

Iain J. McFadyen, Thomas G. Metzger, M.Germana Paterlini, and David M. Ferguson

Exploring the unique pharmacology of a novel opioid receptor, ZFOR1, using molecular modeling and the `message–address' concept
Protein Eng. 2001 14: 953-960; doi:10.1093/protein/14.12.953 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Chie Motono, Tairo Oshima, and Akihiko Yamagishi

High thermal stability of 3-isopropylmalate dehydrogenase from Thermus thermophilus resulting from low C_p of unfolding
Protein Eng. 2001 14: 961-966; doi:10.1093/protein/14.12.961 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Takumi Koshiba, Yoshihiro Kobashigawa, Makoto Demura, and Katsutoshi Nitta

Energetics of three-state unfolding of a protein: canine milk lysozyme
Protein Eng. 2001 14: 967-974; doi:10.1093/protein/14.12.967 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Naoto Ohtani, Mitsuru Haruki, Masaaki Morikawa, and Shigenori Kanaya

Heat labile ribonuclease HI from a psychrotrophic bacterium: gene cloning, characterization and site-directed mutagenesis
Protein Eng. 2001 14: 975-982; doi:10.1093/protein/14.12.975 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

D.Andrew James, Darcy C. Burns, and G.Andrew Woolley

Kinetic characterization of ribonuclease S mutants containing photoisomerizable phenylazophenylalanine residues
Protein Eng. 2001 14: 983-991; doi:10.1093/protein/14.12.983 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Rachel B. Kapust, József Tözsér, Jeffrey D. Fox, D.Eric Anderson, Scott Cherry, Terry D. Copeland, and David S. Waugh

Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency
Protein Eng. 2001 14: 993-1000; doi:10.1093/protein/14.12.993 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Yiming Ye, Hsiau-Wei Lee, Wei Yang, Sarah J. Shealy, Anna L. Wilkins, Zhi-ren Liu, Ivan Torshin, Robert Harrison, Robert Wohlhueter, and Jenny J. Yang

Metal binding affinity and structural properties of an isolated EF-loop in a scaffold protein
Protein Eng. 2001 14: 1001-1013; doi:10.1093/protein/14.12.1001 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Susann Taudte, Hong Xin, Anthony J. Bell, Jr, and Neville R. Kallenbach

Interactions between HMG boxes
Protein Eng. 2001 14: 1015-1023; doi:10.1093/protein/14.12.1015 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Anna M. Wu, Giselle J. Tan, Mark A. Sherman, Patrick Clarke, Tove Olafsen, Stephen J. Forman, and Andrew A. Raubitschek

Multimerization of a chimeric anti-CD20 single-chain Fv-Fc fusion protein is mediated through variable domain exchange
Protein Eng. 2001 14: 1025-1033; doi:10.1093/protein/14.12.1025 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Jerry Thompson, Scott Stavrou, Marla Weetall, J.Mark Hexham, Mary Ellen Digan, Zhuri Wang, Jung Hee Woo, Yongjun Yu, Askale Mathias, Yuan Yi Liu, Shenglin Ma, Irina Gordienko, Philip Lake, and David M. Neville, Jr

Improved binding of a bivalent single-chain immunotoxin results in increased efficacy for in vivo T-cell depletion
Protein Eng. 2001 14: 1035-1041; doi:10.1093/protein/14.12.1035 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Christian Heinis, Adrian Huber, Salvatore Demartis, Julian Bertschinger, Samu Melkko, Luisa Lozzi, Paolo Neri, and Dario Neri

Selection of catalytically active biotin ligase and trypsin mutants by phage display
Protein Eng. 2001 14: 1043-1052; doi:10.1093/protein/14.12.1043 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

ERRATUM:

Alex C.W. May

Optimal classification of protein sequences and selection of representative sets from multiple alignments: application to homologous families and lessons for structural genomics
Protein Eng. 2001 14: 1053; doi:10.1093/protein/14.12.1053 [Extract] [FREE Full Text] [PDF] [Request Permissions]  

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