Protein Engineering Design and Selection

ORIGINAL ARTICLE:

Giulio Gianese, Patrick Argos, and Stefano Pascarella

Structural adaptation of enzymes to low temperatures
Protein Eng. 2001 14: 141-148; doi:10.1093/protein/14.3.141 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Gloria Saab-Rincón, Victor Rivelino Juárez, Joel Osuna, Filiberto Sánchez, and Xavier Soberón

Different strategies to recover the activity of monomeric triosephosphate isomerase by directed evolution
Protein Eng. 2001 14: 149-155; doi:10.1093/protein/14.3.149 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Klaus H. Dittmann, Nuri Gueven, Claus Mayer, and Hans-Peter Rodemann

Characterization of the amino acids essential for the photo- and radioprotective effects of a Bowman–Birk protease inhibitor-derived nonapeptide
Protein Eng. 2001 14: 157-160; doi:10.1093/protein/14.3.157 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

H. Chirakkal, G.C. Ford, and A. Moir

Analysis of a conserved hydrophobic pocket important for the thermostability of Bacillus pumilus chloramphenicol acetyltransferase (CAT-86)
Protein Eng. 2001 14: 161-166; doi:10.1093/protein/14.3.161 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Irina A. Kataeva, David L. Blum, Xin-Liang Li, and Lars G. Ljungdahl

Do domain interactions of glycosyl hydrolases from Clostridium thermocellum contribute to protein thermostability?
Protein Eng. 2001 14: 167-172; doi:10.1093/protein/14.3.167 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

María Pilar Irún, Susana Maldonado, and Javier Sancho

Stabilization of apoflavodoxin by replacing hydrogen-bonded charged Asp or Glu residues by the neutral isosteric Asn or Gln
Protein Eng. 2001 14: 173-181; doi:10.1093/protein/14.3.173 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Christoph Heiring and Yves A. Muller

Folding screening assayed by proteolysis: application to various cystine deletion mutants of vascular endothelial growth factor
Protein Eng. 2001 14: 183-188; doi:10.1093/protein/14.3.183 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

S.A. Cain, D.M. Williams, V. Harris, and P.N. Monk

Selection of novel ligands from a whole-molecule randomly mutated C5a library
Protein Eng. 2001 14: 189-193; doi:10.1093/protein/14.3.189 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Sofia Casares, Constantin A. Bona, and Teodor-D. Brumeanu

Enzymatically mediated engineering of multivalent MHC class II–peptide chimeras
Protein Eng. 2001 14: 195-200; doi:10.1093/protein/14.3.195 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Chitkala Kalidas, Lokesh Joshi, and Carl Batt

Characterization of glycosylated variants of ß-lactoglobulin expressed in Pichia pastoris
Protein Eng. 2001 14: 201-207; doi:10.1093/protein/14.3.201 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

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