Protein Engineering Design and Selection

ORIGINAL ARTICLE:

Alex C. W. May

Optimal classification of protein sequences and selection of representative sets from multiple alignments: application to homologous families and lessons for structural genomics
Protein Eng. 2001 14: 209-217; doi:10.1093/protein/14.4.209 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

S. Balaji and N. Srinivasan

Use of a database of structural alignments and phylogenetic trees in investigating the relationship between sequence and structural variability among homologous proteins
Protein Eng. 2001 14: 219-226; doi:10.1093/protein/14.4.219 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Andrew J. Jennings, Colin M. Edge, and Michael J.E. Sternberg

An approach to improving multiple alignments of protein sequences using predicted secondary structure
Protein Eng. 2001 14: 227-231; doi:10.1093/protein/14.4.227 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Anne Lebrun, Richard Lavery, and Harel Weinstein

Modeling multi-component protein–DNA complexes: the role of bending and dimerization in the complex of p53 dimers with DNA
Protein Eng. 2001 14: 233-243; doi:10.1093/protein/14.4.233 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Richard J. Stewart, Jose N. Varghese, Thomas P.J. Garrett, Peter B. Høj, and Geoffrey B. Fincher

Mutant barley (13,14)-ß-glucan endohydrolases with enhanced thermostability
Protein Eng. 2001 14: 245-253; doi:10.1093/protein/14.4.245 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

E. Pedone, M. Saviano, M. Rossi, and S. Bartolucci

A single point mutation (Glu85Arg) increases the stability of the thioredoxin from Escherichia coli
Protein Eng. 2001 14: 255-260; doi:10.1093/protein/14.4.255 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Simon Delagrave, Dennis J. Murphy, Jennifer L. Rittenhouse Pruss, Anthony M. Maffia, III, Barry L. Marrs, Edward J. Bylina, William J. Coleman, Christina L. Grek, Michael R. Dilworth, Mary M. Yang, and Douglas C. Youvan

Application of a very high-throughput digital imaging screen to evolve the enzyme galactose oxidase
Protein Eng. 2001 14: 261-267; doi:10.1093/protein/14.4.261 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Nigel A. Turner, Duncan J. H. Gaskin, Asutosh T. Yagnik, Jennifer A. Littlechild, and Evgeny N. Vulfson

Enantioselectivity of recombinant Rhizomucor miehei lipase in the ring opening of oxazolin-5(4H)-ones
Protein Eng. 2001 14: 269-278; doi:10.1093/protein/14.4.269 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Zinaida Kravchuk, Yaroslav Tsybovsky, Marianne Koivulehto, Alexander Vlasov, Alexander Chumanevich, Natalia Battchikova, Sergey Martsev, and Timo Korpela

Truncated aspartate aminotransferase from alkalophilic Bacillus circulans with deletion of N-terminal 32 amino acids is a non-functional monomer in a partially structured state
Protein Eng. 2001 14: 279-285; doi:10.1093/protein/14.4.279 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

M.K. Short, P.D. Jeffrey, A. Demirjian, and M.N. Margolies

A single H:CDR3 residue in the anti-digoxin antibody 26-10 modulates specificity for C16-substituted digoxin analogs
Protein Eng. 2001 14: 287-296; doi:10.1093/protein/14.4.287 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Yves Nominé, Tutik Ristriani, Cécile Laurent, Jean-François Lefèvre, Étienne Weiss, and Gilles Travé

A strategy for optimizing the monodispersity of fusion proteins: application to purification of recombinant HPV E6 oncoprotein
Protein Eng. 2001 14: 297-305; doi:10.1093/protein/14.4.297 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

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