Protein Engineering Design and Selection

ORIGINAL ARTICLE:

Florencio Pazos and Alfonso Valencia

Similarity of phylogenetic trees as indicator of protein–protein interaction
Protein Eng. 2001 14: 609-614; doi:10.1093/protein/14.9.609 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Oskar Markovic and Stefan Janecek

Pectin degrading glycoside hydrolases of family 28: sequence-structural features, specificities and evolution
Protein Eng. 2001 14: 615-631; doi:10.1093/protein/14.9.615 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Takuyo Aita, Masahiro Iwakura, and Yuzuru Husimi

A cross-section of the fitness landscape of dihydrofolate reductase
Protein Eng. 2001 14: 633-638; doi:10.1093/protein/14.9.633 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Oliviero Carugo, Shanyun Lu, Jingchu Luo, Xiaocheng Gu, Songping Liang, Stefan Strobl, and Sándor Pongor

Structural analysis of free and enzyme-bound amaranth -amylase inhibitor: classification within the knottin fold superfamily and analysis of its functional flexibility
Protein Eng. 2001 14: 639-646; doi:10.1093/protein/14.9.639 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Geun-Joong Kim, Young-Hoon Cheon, Min-Soon Park, Hee-Sung Park, and Hak-Sung Kim

Generation of protein lineages with new sequence spaces by functional salvage screen
Protein Eng. 2001 14: 647-654; doi:10.1093/protein/14.9.647 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Ursula Gerike, Michael J. Danson, and David W. Hough

Cold-active citrate synthase: mutagenesis of active-site residues
Protein Eng. 2001 14: 655-661; doi:10.1093/protein/14.9.655 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Shin-ichi Sakasegawa, Hideki Takehara, Issei Yoshioka, Mamoru Takahashi, Yoshitaka Kagimoto, Hideo Misaki, Haruhiko Sakuraba, and Toshihisa Ohshima

Increasing the thermostability of Flavobacterium meningosepticum glycerol kinase by changing Ser329 to Asp in the subunit interface region
Protein Eng. 2001 14: 663-667; doi:10.1093/protein/14.9.663 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Takuji Tanaka and Rickey Y. Yada

N-terminal portion acts as an initiator of the inactivation of pepsin at neutral pH
Protein Eng. 2001 14: 669-674; doi:10.1093/protein/14.9.669 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Dapeng Sun, Limei H. Jones, F.Scott Mathews, and Victor L. Davidson

Active-site residues are critical for the folding and stability of methylamine dehydrogenase
Protein Eng. 2001 14: 675-681; doi:10.1093/protein/14.9.675 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

A.A. Vinogradov, E.V. Kudryashova, V.Ya. Grinberg, N.V. Grinberg, T.V. Burova, and A.V. Levashov

The chemical modification of -chymotrypsin with both hydrophobic and hydrophilic compounds stabilizes the enzyme against denaturation in water–organic media
Protein Eng. 2001 14: 683-689; doi:10.1093/protein/14.9.683 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Andrew M. Coley, Naomi V. Campanale, Joanne L. Casey, Anthony N. Hodder, Pauline E. Crewther, Robin F. Anders, Leann M. Tilley, and Michael Foley

Rapid and precise epitope mapping of monoclonal antibodies against Plasmodium falciparum AMA1 by combined phage display of fragments and random peptides
Protein Eng. 2001 14: 691-698; doi:10.1093/protein/14.9.691 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Lianhong Sun, Ioanna P. Petrounia, Makoto Yagasaki, Geethani Bandara, and Frances H. Arnold

Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution
Protein Eng. 2001 14: 699-704; doi:10.1093/protein/14.9.699 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Nozomi Koganesawa, Tomoyasu Aizawa, Kazuo Masaki, Atsushi Matsuura, Taisuke Nimori, Hisanori Bando, Keiichi Kawano, and Katsutoshi Nitta

Construction of an expression system of insect lysozyme lacking thermal stability: the effect of selection of signal sequence on level of expression in the Pichia pastoris expression system
Protein Eng. 2001 14: 705-710; doi:10.1093/protein/14.9.705 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Malin Gustavsson, Janne Lehtiö, Stuart Denman, Tuula T. Teeri, Karl Hult, and Mats Martinelle

Stable linker peptides for a cellulose-binding domain–lipase fusion protein expressed in Pichia pastoris
Protein Eng. 2001 14: 711-715; doi:10.1093/protein/14.9.711 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

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