Protein Engineering Design and Selection

ORIGINAL ARTICLES:

Christilyn P. Graff, Kerry Chester, Richard Begent, and K.Dane Wittrup

Directed evolution of an anti-carcinoembryonic antigen scFv with a 4-day monovalent dissociation half-time at 37°C
PEDS Advance Access published on April 28, 2004
Protein Engineering, Design and Selection 2004 17: 293-304; doi:10.1093/protein/gzh038 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Takeshi Tenno, Natsuko Goda, Yukihiro Tateishi, Hidehito Tochio, Masaki Mishima, Hidenori Hayashi, Masahiro Shirakawa, and Hidekazu Hiroaki

High-throughput construction method for expression vector of peptides for NMR study suited for isotopic labeling
PEDS Advance Access published on May 27, 2004
Protein Engineering, Design and Selection 2004 17: 305-314; doi:10.1093/protein/gzh044 [Abstract] [FREE Full Text] [PDF] [Supplementary data] [Request Permissions]  

Tove Olafsen, Giselle J. Tan, Chia-wei Cheung, Paul J. Yazaki, Jinha M. Park, John E. Shively, Lawrence E. Williams, Andrew A. Raubitschek, Michael F. Press, and Anna M. Wu

Characterization of engineered anti-p185^HER-2 (scFv-C_H3)₂ antibody fragments (minibodies) for tumor targeting
PEDS Advance Access published on June 8, 2004
Protein Engineering, Design and Selection 2004 17: 315-323; doi:10.1093/protein/gzh040 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Hana Im, Myung-Jeom Ryu, and Myeong-Hee Yu

Engineering thermostability in serine protease inhibitors
PEDS Advance Access published on May 4, 2004
Protein Engineering, Design and Selection 2004 17: 325-331; doi:10.1093/protein/gzh036 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Philip J. Ganz, Mark D. Bauer, Yiping Sun, Angela M. Fieno, Raymond A. Grant, Paul E. Correa, Michael Laskowski, Jr, and Charles W. Saunders

Stabilized variant of Streptomyces subtilisin inhibitor and its use in stabilizing subtilisin BPN'
PEDS Advance Access published on June 8, 2004
Protein Engineering, Design and Selection 2004 17: 333-339; doi:10.1093/protein/gzh045 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

J. Xiang and N.S. Sampson

Library screening studies to investigate substrate specificity in the reaction catalyzed by cholesterol oxidase
PEDS Advance Access published on May 10, 2004
Protein Engineering, Design and Selection 2004 17: 341-348; doi:10.1093/protein/gzh041 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Jannick Dyrløv Bendtsen, Lars Juhl Jensen, Nikolaj Blom, Gunnar von Heijne, and Søren Brunak

Feature-based prediction of non-classical and leaderless protein secretion
PEDS Advance Access published on April 28, 2004
Protein Engineering, Design and Selection 2004 17: 349-356; doi:10.1093/protein/gzh037 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Fabrice Le Gall, Uwe Reusch, Melvyn Little, and Sergey M. Kipriyanov

Effect of linker sequences between the antibody variable domains on the formation, stability and biological activity of a bispecific tandem diabody
PEDS Advance Access published on May 4, 2004
Protein Engineering, Design and Selection 2004 17: 357-366; doi:10.1093/protein/gzh039 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Andrea Passerini and Paolo Frasconi

Learning to discriminate between ligand-bound and disulfide-bound cysteines
PEDS Advance Access published on May 27, 2004
Protein Engineering, Design and Selection 2004 17: 367-373; doi:10.1093/protein/gzh042 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

I. Kursula, M. Salin, J. Sun, B.V. Norledge, A.M. Haapalainen, N.S. Sampson, and R.K. Wierenga

Understanding protein lids: structural analysis of active hinge mutants in triosephosphate isomerase
PEDS Advance Access published on May 27, 2004
Protein Engineering, Design and Selection 2004 17: 375-382; doi:10.1093/protein/gzh048 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Z. Oliynyk, L. Briseño-Roa, T. Janowitz, P. Sondergeld, and A.R. Fersht

Designing a metal-binding site in the scaffold of Escherichia coli KDO8PS
PEDS Advance Access published on May 27, 2004
Protein Engineering, Design and Selection 2004 17: 383-390; doi:10.1093/protein/gzh046 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Mark A. Olson, John H. Carra, Virginia Roxas-Duncan, Robert W. Wannemacher, Leonard A. Smith, and Charles B. Millard

Finding a new vaccine in the ricin protein fold
PEDS Advance Access published on June 8, 2004
Protein Engineering, Design and Selection 2004 17: 391-397; doi:10.1093/protein/gzh043 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

Aitziber L. Cortajarena, Tommi Kajander, Weilan Pan, Melanie J. Cocco, and Lynne Regan

Protein design to understand peptide ligand recognition by tetratricopeptide repeat proteins
PEDS Advance Access published on May 27, 2004
Protein Engineering, Design and Selection 2004 17: 399-409; doi:10.1093/protein/gzh047 [Abstract] [FREE Full Text] [PDF] [Request Permissions]  

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