Protein Engineering Design and Selection

ORIGINAL ARTICLES:

Susanne Aileen Funke, Nikolaj Otte, Thorsten Eggert, Marco Bocola, Karl-Erich Jaeger, and Walter Thiel

Combination of computational prescreening and experimental library construction can accelerate enzyme optimization by directed evolution
PEDS Advance Access published on October 3, 2005
Protein Engineering, Design and Selection 2005 18: 509-514; doi:10.1093/protein/gzi062 [Abstract] [Full Text] [PDF] [Supplementary data] [Request Permissions]  

Kenji Fukuda, Malene H. Jensen, Richard Haser, Nushin Aghajari, and Birte Svensson

Biased mutagenesis in the N-terminal region by degenerate oligonucleotide gene shuffling enhances secretory expression of barley -amylase 2 in yeast
PEDS Advance Access published on September 9, 2005
Protein Engineering, Design and Selection 2005 18: 515-526; doi:10.1093/protein/gzi057 [Abstract] [Full Text] [PDF] [Request Permissions]  

Jane M. Weaver-Feldhaus, Keith D. Miller, Michael J. Feldhaus, and Robert W. Siegel

Directed evolution for the development of conformation-specific affinity reagents using yeast display
PEDS Advance Access published on September 26, 2005
Protein Engineering, Design and Selection 2005 18: 527-536; doi:10.1093/protein/gzi060 [Abstract] [Full Text] [PDF] [Request Permissions]  

Kristina Jonas, Erhard Van Der Vries, Mikael T.I. Nilsson, and Mikael Widersten

Isolation of novel single-chain Cro proteins targeted for binding to the bcl-2 transcription initiation site by repertoire selection and subunit combinatorics
PEDS Advance Access published on September 26, 2005
Protein Engineering, Design and Selection 2005 18: 537-546; doi:10.1093/protein/gzi058 [Abstract] [Full Text] [PDF] [Request Permissions]  

Daniel Otzen

Antagonism, non-native interactions and non-two-state folding in S6 revealed by double-mutant cycle analysis
PEDS Advance Access published on October 17, 2005
Protein Engineering, Design and Selection 2005 18: 547-557; doi:10.1093/protein/gzi063 [Abstract] [Full Text] [PDF] [Request Permissions]  

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