The structure of protein complexes can be determined based on structural knowledge of the individual protein components using a hybrid biochemical/computational approach. In the example shown here, the structure of carboxypeptidase A and its mammalian inhibitor latexin has been modelled using this approach. Simple rigid body docking was carried out with the help of distance constraints derived from chemical cross-linking.The figure shows backbone traces of the 1000 top-scoring models of latexin (red) docked around carboxypeptidase A (blue), as well as the crystallographically determined position of latexin (green). For details, see Mouradov et al., pages 9–16.
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