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Mammalian cytochromes P450 2B metabolize drugs, carcinogens, and industrial contaminants, and are therefore of commercial importance. Here, directed evolution has been used to engineer P450 2B1 for enhanced catalytic tolerance to temperature and organic solvent. This figure represents a homology model of the evolved 2B1 enzyme based on an x-ray crystal structure of P450 2B4 (pdb:1SUO), illustrating the location and the nature of the mutants that show enhanced catalytic tolerance to temperature (L295H) and dimethyl sulfoxide (K236I/D257N/L295H). These mutations were created in the V183L/F202L/L209A/S334P background. For further details see: Engineering mammalian cytochrome P450 2B1 by directed evolution for enhanced catalytic tolerance to temperature and dimethyl sulfoxide by Kumar
et al. (pp.
547–554).
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