Protein Engineering Design and Selection

Commentary:

Leonard Presta

Protein structure analysis and development of databases
Protein Eng. 1989 2: 395-397; doi:10.1093/protein/2.6.395 [PDF] [Request Permissions]  

ORIGINAL ARTICLES:

Alec D. Tucker, Daniel Baty, Michael W. Parker, Franc Pattus, Claude Lazdunski, and Demetrius Tsernoglou

Crystallographic phases through genetic engineering: experiences with colicin A
Protein Eng. 1989 2: 399-405; doi:10.1093/protein/2.6.399 [Abstract] [PDF] [Request Permissions]  

Richard Engh, Hartmut Löbermann, Monika Schneider, Georg Wiegand, Robert Huber, and Carl-Bertil Laurell

The S variant of human ₁-antitrypsin, structure and implications for function and metabolism
Protein Eng. 1989 2: 407-415; doi:10.1093/protein/2.6.407 [Abstract] [PDF] [Request Permissions]  

R. de Llorens, C. Arús, X. Parés, and C.M. Cuchillo

Chemical and computer graphics studies on the topography of the ribonuclease A active site cleft. A model of the enzymepentanucleotide substrate complex
Protein Eng. 1989 2: 417-429; doi:10.1093/protein/2.6.417 [Abstract] [PDF] [Request Permissions]  

Suhail A. Islam and Michael J.E. Sternberg

A relational database of protein structures designed for flexible enquiries about conformation
Protein Eng. 1989 2: 431-442; doi:10.1093/protein/2.6.431 [Abstract] [PDF] [Request Permissions]  

O.B. Ptitsyn and A.V. Finkelstein

Prediction of protein secondary structure based on physical theory. Histones
Protein Eng. 1989 2: 443-447; doi:10.1093/protein/2.6.443 [Abstract] [PDF] [Request Permissions]  

Per F. Nielsen, Peter Roepstorff, Ib G. Clausen, Ejner B. Jensen, Ib Jonassen, Allan Svendsen, Per Balschmidt, and Finn B. Hansen

Plasma desorption mass spectrometry, an analytical tool in protein engineering: characterization of modified insulins
Protein Eng. 1989 2: 449-457; doi:10.1093/protein/2.6.449 [Abstract] [PDF] [Request Permissions]  

E. Degryse, M. Acker, G. Defreyn, A. Bernat, J.P. Maffrand, C. Roitsch, and M. Courtney

Point mutations modifying the thrombin inhibition kinetics and antithrombotic activity in vivo of recombinant hirudin
Protein Eng. 1989 2: 459-465; doi:10.1093/protein/2.6.459 [Abstract] [PDF] [Request Permissions]  

Oscar P. Kuipers, Ruud Dijkman, Cornelieke E.G.M. Pals, Hubertus M. Verheij, and Gerard H.de Haas

Evidence for the involvement of tyrosine-69 in the control of stereospecificity of porcine pancreatic phospholipase A₂
Protein Eng. 1989 2: 467-471; doi:10.1093/protein/2.6.467 [Abstract] [PDF] [Request Permissions]  

Sylvie Blanchin-Roland and Jean-Michel Masson

Protein secretion controlled by a synthetic gene in Escherichia coli
Protein Eng. 1989 2: 473-480; doi:10.1093/protein/2.6.473 [Abstract] [PDF] [Request Permissions]  

Akiko Saito, Shinkichi Honda, Tatsunari Nishi, Masamichi Koike, Kei Okazaki, Seiga Itoh, and Moriyuki Sato

High level expression of a synthetic gene coding for IgG-binding domain B of Staphylococcal protein A
Protein Eng. 1989 2: 481-487; doi:10.1093/protein/2.6.481 [Abstract] [PDF] [Request Permissions]  

PROTOCOL:

D.H. Thomas, A. Rob, and D.W. Rice

A novel dialysis procedure for the crystallization of proteins
Protein Eng. 1989 2: 489-491; doi:10.1093/protein/2.6.489 [Abstract] [PDF] [Request Permissions]  

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