cover illustration A heat-inducible autolytic vector was constructed for quick and consistent lysis of E. coli cells in situ for high throughput screening. Genes for Aspergillus fumigattus amadoriase II and Bacillus subtilis lipase A were inserted into the vector and expressed in BL 21 cells. Upon heat treatment at 42°C for 30 min, uniform colors developed for the amadoriase on a nitrocellulose membrane (A) containing glycated lysine, and the lipase on an agar plate containing rhodamine B and olive oil (C). Control experiments with similar expression vectors lacking the autolytic unit showed little color developments for either the amadoriase (B) or the lipase (D). For further details please see Cai et al. pp. 681-687.
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