Protein Engineering Design and Selection

COMMENTARY:

I. Tuñón, E. Silla, and J.L. Pascual-Ahuir

Molecular surface area and hydrophobic effect
Protein Eng. 1992 5: 715-716; doi:10.1093/protein/5.8.715 [PDF] [Request Permissions]  

ORIGINAL ARTICLES:

Adrian S. Morris, Narmada Thanki, and Julia M. Goodfellow

Hydration of amino acid side chains: dependence on secondary structure
Protein Eng. 1992 5: 717-728; doi:10.1093/protein/5.8.717 [Abstract] [PDF] [Request Permissions]  

Samuel Karlin, B.Edwin Blaisdell, and Philipp Bucher

Quantile distributions of amino acid usage in protein classes
Protein Eng. 1992 5: 729-738; doi:10.1093/protein/5.8.729 [Abstract] [PDF] [Request Permissions]  

Costas D. Paliakasis and Michael Kokkinidis

Relationships between sequence and structure for the four--helix bundle tertiary motif in proteins
Protein Eng. 1992 5: 739-748; doi:10.1093/protein/5.8.739 [Abstract] [PDF] [Request Permissions]  

Annalisa Pastore, Raffaele De Francesco, Maria Antonietta Castiglione Morelli, Dominique Nalis, and Riccardo Cortese

The dimerization domain of LFB1/HNF1 related transcription factors: a hidden four helix bundle?
Protein Eng. 1992 5: 749-757; doi:10.1093/protein/5.8.749 [Abstract] [PDF] [Request Permissions]  

Yuto Komeiji, Masami Uebayasi, Jun-ichiro Someya, and Ichiro Yamato

Free energy perturbation study on a Trp-binding mutant (Ser⁸⁸ Cys) of the trp-repressor
Protein Eng. 1992 5: 759-767; doi:10.1093/protein/5.8.759 [Abstract] [PDF] [Request Permissions]  

Helmut K.W. Kallwass, Witold K. Surewicz, Wendy Parris, Emma L.A. Macfarlane, Marcel A. Luyten, Cyril M. Kay, Marvin Gold, and J.Bryan Jones

Single amino acid substitutions can further increase the stability of a thermophilic L-lactate dehydrogenase
Protein Eng. 1992 5: 769-774; doi:10.1093/protein/5.8.769 [Abstract] [PDF] [Request Permissions]  

Youngsoo Kim and Jon D. Robertus

Analysis of several key active site residues of ricin A chain by mutagenesis and X-ray crystallography
Protein Eng. 1992 5: 775-779; doi:10.1093/protein/5.8.775 [Abstract] [PDF] [Request Permissions]  

Vladimir N. Uversky, Vladimir V. Leontiev, and Anatoly T. Gudkov

Triple point mutation Asp10 His, Asn101 Asp, Argl48 Ser in T4 phage lysozyme leads to the molten globule
Protein Eng. 1992 5: 781-783; doi:10.1093/protein/5.8.781 [Abstract] [PDF] [Request Permissions]  

C. Derst, J. Henseling, and K.H. Röhm

Probing the role of threonine and serine residues of E.coli asparaginase II by site-specific mutagenesis
Protein Eng. 1992 5: 785-789; doi:10.1093/protein/5.8.785 [Abstract] [PDF] [Request Permissions]  

M. Iwakura and C.R. Matthews

Construction and characterization of a single polypeptide chain containing two enzymatically active dihydrofolate reductase domains
Protein Eng. 1992 5: 791-796; doi:10.1093/protein/5.8.791 [Abstract] [PDF] [Request Permissions]  

Kim Ry Hejnaes, Stephen Bayne, Leif Nørskov, Hans Holmegaard, H.H. Sørensen, Johannes Thomsen, Lauge Schäffer, Axel Wollmer, and Lars Skriver

Development of an optimized refolding process for recombinant Ala–Glu–IGF-1
Protein Eng. 1992 5: 797-806; doi:10.1093/protein/5.8.797 [Abstract] [PDF] [Request Permissions]  

K. Adachi, P. Konitzer, C.H. Lai, J. Kim, and S. Surrey

Oxygen binding and other physical properties of human hemoglobin made in yeast
Protein Eng. 1992 5: 807-810; doi:10.1093/protein/5.8.807 [Abstract] [PDF] [Request Permissions]  

S. Cox and L.N. Johnson

Expression of the phosphorylase kinase subunit catalytic domain in Escherichia coli
Protein Eng. 1992 5: 811-819; doi:10.1093/protein/5.8.811 [Abstract] [PDF] [Request Permissions]  

Giacinto Libertini and Alberto Di Donato

Computer-aided gene design
Protein Eng. 1992 5: 821-825; doi:10.1093/protein/5.8.821 [Abstract] [PDF] [Request Permissions]  

PROTOCOL:

Chrisostomos Prodromou and Laurence H. Pearl

Recursive PCR: a novel technique for total gene synthesis
Protein Eng. 1992 5: 827-829; doi:10.1093/protein/5.8.827 [PDF] [Request Permissions]  

Acknowledgements:

Acknowledgements
Protein Eng. 1992 5: 831-832; doi:10.1093/protein/5.8.831 [PDF] [Request Permissions]  

Author index:

Author index
Protein Eng. 1992 5: 839-840; doi:10.1093/protein/5.8.839 [PDF] [Request Permissions]  

Subject index:

Subject index
Protein Eng. 1992 5: 841-845; doi:10.1093/protein/5.8.841 [PDF] [Request Permissions]  

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