Protein Engineering Design and Selection

COMMENTARY:

Krishna Murthy

Molecular astrology: the case of the Myb DNA binding domain
Protein Eng. 1993 6: 129-131; doi:10.1093/protein/6.2.129 [PDF] [Request Permissions]  

REVIEW:

Adrian J. Mulholland, Guy H. Grant, and W.Graham Richards

Computer modelling of enzyme catalysed reaction mechanisms
Protein Eng. 1993 6: 133-147; doi:10.1093/protein/6.2.133 [PDF] [Request Permissions]  

ORIGINAL ARTICLES:

Christophe Romier, Jean-Marie Bernassau, Christian Cambillau, and Hervé Darbon

Solution structure of human corticotropin releasing factor by ¹H NMR and distance geometry with restrained molecular dynamics
Protein Eng. 1993 6: 149-156; doi:10.1093/protein/6.2.149 [Abstract] [PDF] [Request Permissions]  

Chrislaine Martinez, Pieter de Geus, Patrick Stanssens, Marc Lauwereys, and Christian Cambillau

Engineering cysteine mutants to obtain crystallographic phases with a cutinase from Fusarium solani pisi
Protein Eng. 1993 6: 157-165; doi:10.1093/protein/6.2.157 [Abstract] [PDF] [Request Permissions]  

T.K. Attwood and J.B.C. Findlay

Design of a discriminating fingerprint for G-protein-coupled receptors
Protein Eng. 1993 6: 167-176; doi:10.1093/protein/6.2.167 [Abstract] [PDF] [Request Permissions]  

Anna-Marja Hoffrén, Markku Saloheimo, Pamela Thomas, John P. Overington, Mark S. Johnson, Jonathan K.C. Knowles, and Tom L. Blundell

Modelling of the lignin peroxidase LIII of Phlebia radiata: use of a sequence template generated from a 3-D structure
Protein Eng. 1993 6: 177-182; doi:10.1093/protein/6.2.177 [Abstract] [PDF] [Request Permissions]  

Tadashi Ueda, Tomohiro Tamura, Yoshitake Maeda, Yoshio Hashimoto, Takeyoshi Miki, Hidenori Yamada, and Taiji Imoto

Stabilization of lysozyme by the introduction of Gly-Pro sequence
Protein Eng. 1993 6: 183-187; doi:10.1093/protein/6.2.183 [Abstract] [PDF] [Request Permissions]  

Kinji Miyashita, Mikiko Kusumi, Ryutaro Utsumi, Satoshi Katayama, Manjiro Noda, Tohru Komano, and Nobukatsu Satoh

Site-directed mutagenesis of the putative active site residues of 3C proteinase of coxsackievirus B3: evidence of a functional relationship with trypsin-like serine proteinases
Protein Eng. 1993 6: 189-193; doi:10.1093/protein/6.2.189 [Abstract] [PDF] [Request Permissions]  

Koichi Uegaki, Masahiro Shirakawa, Takashi Fujita, Tadatsugu Taniguchi, and Yoshimasa Kyogoku

Characterization of the DNA binding domain of the mouse IRF-2 protein
Protein Eng. 1993 6: 195-200; doi:10.1093/protein/6.2.195 [Abstract] [PDF] [Request Permissions]  

Rutger J. van Rooijen, Koen J. Dechering, C. Niek, J. Wilmink, and Willem M. de Vos

Lysines 72, 80 and 213 and aspartic acid 210 of the Lactococcus lactis LacR repressor are involved in the response to the inducer tagatose-6-phosphate leading to induction of lac operon expression
Protein Eng. 1993 6: 201-206; doi:10.1093/protein/6.2.201 [Abstract] [PDF] [Request Permissions]  

Paul J. Loida and Stephen G. Sligar

Engineering cytochrome P-450_cam to increase the stereospecificity and coupling of aliphatic hydroxylation
Protein Eng. 1993 6: 207-212; doi:10.1093/protein/6.2.207 [Abstract] [PDF] [Request Permissions]  

Thierry Vernet, Jean Chatellier, Daniel C. Tessier, and David Y. Thomas

Expression of functional papain precursor in Saccharomyces cerevisiae: rapid screening of mutants
Protein Eng. 1993 6: 213-219; doi:10.1093/protein/6.2.213 [Abstract] [PDF] [Request Permissions]  

Andrew P. Hinck, William F. Walkenhorst, William M. Westler, Soheui Choe, and John L. Markley

Overexpression and purification of avian ovomucoid third domains in Escherichia coli
Protein Eng. 1993 6: 221-227; doi:10.1093/protein/6.2.221 [Abstract] [PDF] [Request Permissions]  

PROTOCOL:

Simon J. Davis, Michael J. Puklavec, David A. Ashford, Karl Harlos, E. Yvonne Jones, David I. Stuart, and Alan F. Williams

Expression of soluble recombinant glycoproteins with predefined glycosylation: application to the crystallization of the T-cell glycoprotein CD2
Protein Eng. 1993 6: 229-232; doi:10.1093/protein/6.2.229 [PDF] [Request Permissions]  

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