Protein Engineering Design and Selection

ORIGINAL ARTICLES:

W.R. Rypniewski, S. Hastrup, Ch. Betzel, M. Dauter, Z. Dauter, G. Papendorf, S. Branner, and K.S. Wilson

The sequence and X-ray structure of the trypsin from Fusarium oxysporum
Protein Eng. 1993 6: 341-348; doi:10.1093/protein/6.4.341 [Abstract] [PDF] [Request Permissions]  

Simon Weston and Dietrich Suck

X-ray structures of two single-residue mutants of DNase I: H134Q and Y76A
Protein Eng. 1993 6: 349-357; doi:10.1093/protein/6.4.349 [Abstract] [PDF] [Request Permissions]  

Mark D. Paulsen and Rick L. Ornstein

Substrate mobility in thiocamphor-bound cytochrome P450_cam: an explanation of the conflict between the observed product profile and the X-ray structure
Protein Eng. 1993 6: 359-365; doi:10.1093/protein/6.4.359 [Abstract] [PDF] [Request Permissions]  

Alexei V. Finkelstein and Haruki Nakamura

Weak points of antiparallel ß-sheets. How are they filled up in globular proteins?
Protein Eng. 1993 6: 367-372; doi:10.1093/protein/6.4.367 [Abstract] [PDF] [Request Permissions]  

Yu Luo, Luhua Lai, Xiaojie Xu, and Youqi Tang

Defining topological equivalences in protein structures by means of a dynamic programming algorithm
Protein Eng. 1993 6: 373-376; doi:10.1093/protein/6.4.373 [Abstract] [PDF] [Request Permissions]  

Nathalie Colloc'h, Catherine Etchebest, Etienne Thoreau, Bernard Henrissat, and Jean-Paul Mornon

Comparison of three algorithms for the assignment of secondary structure in proteins: the advantages of a consensus assignment
Protein Eng. 1993 6: 377-382; doi:10.1093/protein/6.4.377 [Abstract] [PDF] [Request Permissions]  

M.A. Andrade, P. Chacón, J.J. Merelo, and F. Morán

Evaluation of secondary structure of proteins from UV circular dichroism spectra using an unsupervised learning neural network
Protein Eng. 1993 6: 383-390; doi:10.1093/protein/6.4.383 [Abstract] [PDF] [Request Permissions]  

Sándor Pongor, Vesna Skerl, Miklós Cserzö, Zsolt Hátsági, György Simon, and Valeria Bevilacqua

The SBASE domain library: a collection of annotated protein segments
Protein Eng. 1993 6: 391-395; doi:10.1093/protein/6.4.391 [Abstract] [PDF] [Request Permissions]  

Andreas P. Heiner, Herman J.C. Berendsen, and Wilfred F. van Gunsteren

Structure prediction of subtilisin BPN' mutants using molecular dynamics methods
Protein Eng. 1993 6: 397-408; doi:10.1093/protein/6.4.397 [Abstract] [PDF] [Request Permissions]  

K. Olesen and M.C. Kielland-Brandt

Altering substrate preference of carboxypeptidase Y by a novel strategy of mutagenesis eliminating wild type background
Protein Eng. 1993 6: 409-415; doi:10.1093/protein/6.4.409 [Abstract] [PDF] [Request Permissions]  

R. Kruse, W. Hengstenberg, W. Beneicke, and H.R. Kalbitzer

Involvement of various amino- and carboxyl-terminal residues in the active site of the histidine-containing protein HPr of the phosphoenolpyruvate-dependent phosphotransferase system of Staphylococcus carnosus: site-directed mutagenesis with the ptsH gene, biochemical characterization and NMR studies of the mutant proteins
Protein Eng. 1993 6: 417-423; doi:10.1093/protein/6.4.417 [Abstract] [PDF] [Request Permissions]  

John A. Chaddock and Lynne M. Roberts

Mutagenesis and kinetic analysis of the active site Glu177 of ricin A-chain
Protein Eng. 1993 6: 425-431; doi:10.1093/protein/6.4.425 [Abstract] [PDF] [Request Permissions]  

Chi-Hon Lee, E-Ching Lee, Shih-Tzer Tsai, Hsing-Jien Kung, Yin-Chang Liu, and Jaulang Hwang

An EGF-pseudomonas exotoxin A recombinant protein with a deletion in toxin binding domain specifically kills EGF receptor bearing cells
Protein Eng. 1993 6: 433-440; doi:10.1093/protein/6.4.433 [Abstract] [PDF] [Request Permissions]  

Lena Cedergren, Roland Andersson, Birger Jansson, Mathias Uhlén, and Björn Nilsson

Mutational analysis of the interaction between staphylococcal protein A and human IgG₁
Protein Eng. 1993 6: 441-448; doi:10.1093/protein/6.4.441 [Abstract] [PDF] [Request Permissions]  

Marja-Leena Laukkanen, Tuula T. Teeri, and Kari Keinänen

Lipid-tagged antibodies: bacterial expression and characterization of a lipoprotein—single-chain antibody fusion protein
Protein Eng. 1993 6: 449-454; doi:10.1093/protein/6.4.449 [Abstract] [PDF] [Request Permissions]  

PROTOCOL:

Ross Barnard

A strategy for optimizing charge reversal mutagenesis of ion pairs in hormone—receptor or enzyme—substrate complexes
Protein Eng. 1993 6: 455-459; doi:10.1093/protein/6.4.455 [PDF] [Request Permissions]  

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