Protein Engineering Design and Selection

REVIEW:

Hiroyuki Toh, Hideo Kubodera, Nobuyuki Nakajima, Tetsuo Sekiya, Naomi Eguchi, Toshiki Tanaka, Yoshihiro Urade, and Osamu Hayaishi

Glutathione-independent prostaglandin D synthase as a lead molecule for designing new functional proteins
Protein Eng. 1996 9: 1067-1082; doi:10.1093/protein/9.12.1067 [PDF] [Request Permissions]  

ORIGINAL ARTICLES:

Keiko Matsuda, Kenji Mizuguchi, Takaaki Nishioka, Hiroaki Kato, Nobuhiro Go, and Jun'ichi Oda

Crystal structure of glutathione synthetase at optimal pH: domain architecture and structural similarity with other proteins
Protein Eng. 1996 9: 1083-1092; doi:10.1093/protein/9.12.1083 [Abstract] [PDF] [Request Permissions]  

Alex C.W. May

Pairwise iterative superposition of distantly related proteins and assessment of the significance of 3-D structural similarity
Protein Eng. 1996 9: 1093-1101; doi:10.1093/protein/9.12.1093 [Abstract] [PDF] [Request Permissions]  

Vladimir Alesker, Ruth Nussinov, and Haim J. Wolfson

Detection of non-topological motifs in protein structures
Protein Eng. 1996 9: 1103-1119; doi:10.1093/protein/9.12.1103 [Abstract] [PDF] [Request Permissions]  

Frank Eisenhaber and Patrick Argos

Hydrophobic regions on protein surfaces: definition based on hydration shell structure and a quick method for their computation
Protein Eng. 1996 9: 1121-1133; doi:10.1093/protein/9.12.1121 [Abstract] [PDF] [Request Permissions]  

Romano T. Kroemer and W. Graham Richards

Homology modeling study of the human interleukin-7 receptor complex
Protein Eng. 1996 9: 1135-1142; doi:10.1093/protein/9.12.1135 [Abstract] [PDF] [Request Permissions]  

Lisa Kuttner-Kondo, M.Edward Medof, William Brodbeck, and Menachem Shoham

Molecular modeling and mechanism of action of human decay-accelerating factor
Protein Eng. 1996 9: 1143-1149; doi:10.1093/protein/9.12.1143 [Abstract] [PDF] [Request Permissions]  

G. Chelvanayagam, I.B. Jakobsen, X. Gao, and S. Easteal

Structural comparison of major histocompatibility complex class I molecules and homology modelling of five distinct human leukocyte antigen-A alleles
Protein Eng. 1996 9: 1151-1164; doi:10.1093/protein/9.12.1151 [Abstract] [PDF] [Request Permissions]  

Chia-Ching Chang and P.C. Huang

Semi-empirical simulation of Zn/Cd binding site preference in the metal binding domains of mammalian metallothionein
Protein Eng. 1996 9: 1165-1172; doi:10.1093/protein/9.12.1165 [Abstract] [PDF] [Request Permissions]  

Masaaki Kurasaki, Tadasu Emoto, Ana Rosa Linde Arias, Masashi Okabe, Futoshi Yamasaki, Shinji Oikawa, and Yutaka Kojima

Independent self-assembly of cadmium-binding -fragment of metallothionein in Escherichia coli without participation of ß-fragment
Protein Eng. 1996 9: 1173-1180; doi:10.1093/protein/9.12.1173 [Abstract] [PDF] [Request Permissions]  

Oene R. Veltman, Gert Vriend, Pieter J. Middelhoven, Bertus van den Burg, Gerard Venema, and Vincent G.H. Eijsink

Analysis of structural determinants of the stability of thermolysinlike proteases by molecular modelling and site-directed mutagenesis
Protein Eng. 1996 9: 1181-1189; doi:10.1093/protein/9.12.1181 [Abstract] [PDF] [Request Permissions]  

Marco Moracci, Luisa Capalbo, Maria Ciaramella, and Mosé Rossi

Identification of two glutamic acid residues essential for catalysis in the ß-glycosidase from the thermoacidophilic archaeon Sulfolobus solfataricus
Protein Eng. 1996 9: 1191-1195; doi:10.1093/protein/9.12.1191 [Abstract] [PDF] [Request Permissions]  

Won Shik Kwon, Nancy A. Da Silva, and James T. Kellis, Jr

Relationship between thermal stability, degradation rate and expression yield of barnase variants in the periplasm of Escherichia coli
Protein Eng. 1996 9: 1197-1202; doi:10.1093/protein/9.12.1197 [Abstract] [PDF] [Request Permissions]  

Richard A. Collins, Sharon M. Kelly, Nicholas C. Price, Linda A. Fothergill-Gilmore, and Hilary Muirhead

Ligand-induced conformational changes in wild-type and mutant yeast pyruvate kinase
Protein Eng. 1996 9: 1203-1210; doi:10.1093/protein/9.12.1203 [Abstract] [PDF] [Request Permissions]  

Yi Li, Meran R.L. Owen, William Cockburn, Izumi Kumagai, and Garry C. Whitelam

Study of antibody–antigen interaction through site-directed mutagenesis of the VH region of a hybrid phage–antibody fragment
Protein Eng. 1996 9: 1211-1217; doi:10.1093/protein/9.12.1211 [Abstract] [PDF] [Request Permissions]  

S. Thennarasu and R. Nagaraj

Specific antimicrobial and hemolytic activities of 18-residue peptides derived from the amino terminal region of the toxin pardaxin
Protein Eng. 1996 9: 1219-1224; doi:10.1093/protein/9.12.1219 [Abstract] [PDF] [Request Permissions]  

C. Wicker-Planquart, S. Canaan, M. Rivière, L. Dupuis, and R. Verger

Expression in insect cells and purification of a catalytically active recombinant human gastric lipase
Protein Eng. 1996 9: 1225-1232; doi:10.1093/protein/9.12.1225 [Abstract] [PDF] [Request Permissions]  

Leslie Lobb, Boguslaw Stec, Evan K. Kantrowitz, Akihito Yamano, Vivian Stojanoff, Ofer Markman, and Martha M. Teeter

Expression, purification and characterization of recombinant crambin
Protein Eng. 1996 9: 1233-1239; doi:10.1093/protein/9.12.1233 [Abstract] [PDF] [Request Permissions]  

Shuichi Kojima, Katsunori Miyoshi, and kin-ichiro Miura

Synthesis of a squash-type protease inhibitor by gene engineering and effects of replacements of conserved hydrophobic amino acid residues on its inhibitory activity
Protein Eng. 1996 9: 1241-1246; doi:10.1093/protein/9.12.1241 [Abstract] [PDF] [Request Permissions]  

Corrigendum:

Chantal Guidi-Rontani

Internalization and translocation of a new chimaeric protein composed of Pseudomonas aeruginosaexotoxin A and mouse dihydrofolate reductase as a model system
Protein Eng. 1996 9: 1247; doi:10.1093/protein/9.12.1247 [Abstract] [PDF] [Request Permissions]  

Acknowledgements:

Acknowledgements
Protein Eng. 1996 9: 1249-1250; doi:10.1093/protein/9.12.1249 [PDF] [Request Permissions]  

Author index:

Author index
Protein Eng. 1996 9: 1251-1252; doi:10.1093/protein/9.12.1251 [PDF] [Request Permissions]  

Subject index:

Subject index
Protein Eng. 1996 9: 1253-1258; doi:10.1093/protein/9.12.1253 [PDF] [Request Permissions]  

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